In 1967, Trinkaus contributed a paper titled, “Procurement, Maintenance and Use of Fundulus Eggs,” to an edited volume about experimental methods in developmental biology (Trinkaus Archives, Box 9, Folder 12). In that review, he lays out the special qualities of Fundulus eggs, which make it a good candidate for studying development. In addition to its transparency, Trinkaus explains that Fundulus eggs are an excellent model to study and observe the process of epiboly because they develop meroblastically. Meroblastic cleavage, or cell division in the early embryo, occurs when only part of the zygote undergoes cell division while the other part, the yolk, serves as nutrition for the embryo. Because of this type of cell division, Fundulus cells are organized in a specific spatial arrangement, which make the cells, the cell aggregates such as cell sheets, and the cell movements during gastrulation easily observable. In this paper, Trinkaus also provides a detailed account of several experimental techniques using Fundulus eggs, including some microsurgical procedures, like dechorionation, fixation techniques, and techniques for developing cell cultures (Trinkaus 1967).